Journal: bioRxiv

Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

doi: 10.64898/2026.02.06.704418

Figure Lengend Snippet: Immunohistochemistry: detection of (A) CD3+ T cells, (B) DBA-lectin+ NK cells and (C) B220+ B cells in uterine tissue sections at 0hr (prior to breakdown, n=4), 12hr (tissue breakdown, n=4-6), 24hr (tissue repair, n=4-5) and 48hr (tissue remodelling, n=4-6) hr after progesterone withdrawal (representative images, scale bar=500µm).

Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

Techniques: Immunohistochemistry

Journal: Microbiology Spectrum

Article Title: Toxoplasma gondii impairs CX3CL1/fractalkine shedding from mouse cortical neurons, leading to microglia activation

doi: 10.1128/spectrum.01074-25

Figure Lengend Snippet: Establishment of an in vitro model of latently infected mouse cortical neuronal cultures. Primary neuronal cultures were established from E18 mouse cortices. After 14 days in vitro , neurons were detectable by β-III-tubulin staining (in red, in A), whereas no astrocytic cells were detected by GFAP staining (in green, in B). Overall neuronal morphology was visualized by Phalloidin staining (in green, in C). Cultures were infected with ME49 tachyzoites at 7 days in vitro and visualized 7 days after (14 days in vitro ). Infection did not disturb neuronal morphology as depicted by neurofilament (NF200, green signal in D and F), and bradyzoite cysts were detected with anti-CST1 antibody (red signal in E). Merged image in F shows the presence of bradyzoite cysts within neurons. Insets in F show higher magnification of the cysts selected with the dashed squares. After 7 days of infection, few tachyzoites were detected in the cultures by SAG1 staining (red signal in G), as well as bradyzoite cysts stained with DBA lectin (green signal in G). Host cell nuclei were detected with DAPI (blue signal A–G). DBA-stained cysts were more abundant at this time point than tachyzoite vacuoles ( H ). Infection did not affect neuronal cellularity ( I ). Each point in ( H ) represents independent cultures, and in ( I ), microscopic fields from two independent cultures. Bars: 50 µm in ( A–C ); 100 µm in ( D–F ), and 175 µm in ( G ).

Article Snippet: T. gondii cysts were alternatively detected using fluorescein-labeled Dolichos Biflorus Agglutinin (DBA) lectin (Vector Laboratories).

Techniques: In Vitro, Infection, Staining